Molecular Characterization of Cancer Associated Fibroblasts in Prostate Cancer.

ABSTRACT:

Background

Stromal components surrounding epithelial cancer cells seem to play a pivotal role during epithelial-to-mesenchymal transition (EMT), tumor invasion, and metastases. To identify the molecular mechanisms underlying tumor-stroma interactions may yield novel therapeutic targets for prostate cancer.

Methods

Gene expression profile of prostate-cancer associated fibroblast (PCAF) and prostate non-cancer associated fibroblast (PNAF) cells isolated from radical prostatectomy was performed by Illumina, analyzed, and further processed by Ingenuity^®: IPA^® software. qRT-PCR was performed on an independent set of 17 PCAF, 12 PNAF, and 12 fibroblast cell lines derived from patients with benign prostatic hyperplasia (BPHF).

Results

Using microarray a

SUBMITTER: Vitale G 

PROVIDER: S-EPMC9221001 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature