Dataset Information

Expression of Zyxin in Non-Small Cell Lung Cancer-A Preliminary Study.

ABSTRACT:

Background

The potential involvement of zyxin (ZYX) in carcinogenesis has been investigated in many cancer types. However, there are a limited number of studies on the role of ZYX in the progression of non-small cell lung cancer (NSCLC). Since lung cancer is one of the most frequently diagnosed carcinomas, the aim of our study was to determine the localization and expression levels of ZYX in NSCLC and to correlate the results with the clinicopathological data.

Materials and methods

The expression of ZYX was assessed in NSCLC cases and in cell lines representing this tumor type. Levels of ZYX were determined in the clinical material using immunohistochemistry (IHC) and Western Blot. Real-time PCR was used to assess ZYX mRNA levels. The expression of ZYX was also checked in NSCLC cell lines using real-time PCR, Western Blot, and immunofluorescence/immunocytochemistry.

Results

The results showed lower levels of ZYX in NSCLC cells compared with control tissues. This trend was observed at the protein and mRNA levels. The assays on the NSCLC model also demonstrated lower levels of ZYX in cancer cells compared with control cells.

Conclusions

The decreased expression of ZYX in NSCLC may indicate a suppressor role of this protein in NSCLC.

SUBMITTER: Partynska A

PROVIDER: S-EPMC9221212 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Publications

Expression of Zyxin in Non-Small Cell Lung Cancer-A Preliminary Study.

Partynska Aleksandra A Gomulkiewicz Agnieszka A Piotrowska Aleksandra A Grzegrzolka Jedrzej J Rzechonek Adam A Ratajczak-Wielgomas Katarzyna K Podhorska-Okolow Marzenna M Dziegiel Piotr P

Biomolecules 20220613 6

<h4>Background</h4>The potential involvement of zyxin (ZYX) in carcinogenesis has been investigated in many cancer types. However, there are a limited number of studies on the role of ZYX in the progression of non-small cell lung cancer (NSCLC). Since lung cancer is one of the most frequently diagnosed carcinomas, the aim of our study was to determine the localization and expression levels of ZYX in NSCLC and to correlate the results with the clinicopathological data.<h4>Materials and methods</h ...[more]

PMID: 35740950

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Dataset Information

Expression of Zyxin in Non-Small Cell Lung Cancer-A Preliminary Study.

Background

Materials and methods

Results

Conclusions

Publications

Expression of Zyxin in Non-Small Cell Lung Cancer-A Preliminary Study.

Similar Datasets

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