HLMSC Secretome Affects Macrophage Activity Differentially Depending on Lung-Mimetic Environments.

ABSTRACT: Mesenchymal stromal cell (MSC)-based therapies for inflammatory diseases rely mainly on the paracrine ability to modulate the activity of macrophages. Despite recent advances, there is scarce information regarding changes of the secretome content attributed to physiomimetic cultures and, especially, how secretome content influence on macrophage activity for therapy. hLMSCs from human donors were cultured on devices developed in house that enabled lung-mimetic strain. hLMSC secretome was analyzed for typical cytokines, chemokines and growth factors. RNA was analyzed for the gene expression of CTGF and CYR61. Human monocytes were differentiated to macrophages and assessed for their phagocytic capacity and for M1/M2 subtypes by the analysis of typical cell surface markers in the presence of h

SUBMITTER: Falcones B 

PROVIDER: S-EPMC9221297 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature