Quantification of Desiccated Extracellular Vesicles by Quartz Crystal Microbalance.

ABSTRACT: Extracellular vesicle (EV) quantification is a procedure through which the biomedical potential of EVs can be used and their biological function can be understood. The number of EVs isolated from cell culture media depends on the cell status and is especially important in studies on cell-to-cell signaling, disease modeling, drug development, etc. Currently, the methods that can be used to quantify isolated EVs are sparse, and each have limitations. In this report, we introduce the application of a quartz crystal microbalance (QCM) as a biosensor for quantifying EVs in a small drop of volatile solvent after it evaporates and leaves desiccated EVs on the surface of the quartz crystal. The shifts in the crystal's resonant frequency were found to obey Sauerbrey's relation for EV quantities up

SUBMITTER: Chernyshev VS 

PROVIDER: S-EPMC9221410 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature