Molecular Cytogenetics Reveals Mosaicism in Human Umbilical Vein Endothelial Cells.

ABSTRACT: Primary human umbilical vein endothelial cells (HUVECs) are consistently the most reliable in vitro model system for studying the inner lining of blood and lymphatic vessels or the endothelium. Primary human cells originate from freshly isolated tissues without genetic manipulation and generally show a modal number of 46 chromosomes with no structural alterations, at least during early passages. We investigated the cytogenetic integrity of HUVECs with conventional (G-banding) and molecular cytogenetic methods (spectral karyotyping (SKY) and fluorescence in situ hybridization (FISH)). Our G-band data shows two X-chromosomes, confirming these HUVECs originate from a female donor. Notably, some cells consistently exhibit an unfamiliar banding pattern on one X chromosome toward the distal end

SUBMITTER: Binz RL 

PROVIDER: S-EPMC9222953 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature