Photoswitchable Fluorescent Proteins: Mechanisms on Ultrafast Timescales.

ABSTRACT: The advancement of super-resolution imaging (SRI) relies on fluorescent proteins with novel photochromic properties. Using light, the reversibly switchable fluorescent proteins (RSFPs) can be converted between bright and dark states for many photocycles and their emergence has inspired the invention of advanced SRI techniques. The general photoswitching mechanism involves the chromophore cis-trans isomerization and proton transfer for negative and positive RSFPs and hydration-dehydration for decoupled RSFPs. However, a detailed understanding of these processes on ultrafast timescales (femtosecond to millisecond) is lacking, which fundamentally hinders the further development of RSFPs. In this review, we summarize the current progress of utilizing various ultrafast electronic

SUBMITTER: Tang L 

PROVIDER: S-EPMC9223536 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature