Structural and Functional Insights into CP2c Transcription Factor Complexes.

ABSTRACT: CP2c, also known as TFCP2, α-CP2, LSF, and LBP-1c, is a prototypic member of the transcription factor (TF) CP2 subfamily involved in diverse ubiquitous and tissue/stage-specific cellular processes and in human malignancies including cancer. Despite its importance, many fundamental regulatory mechanisms of CP2c are still unclear. Here, we uncover unprecedented structural and functional aspects of CP2c using DSP crosslinking and Western blot in addition to conventional methods. We found that a monomeric form of a CP2c homotetramer (tCP2c; [C4]) binds to the known CP2c-binding DNA motif (CNRG-N(5~6)-CNRG), whereas a dimeric form of a CP2c, CP2b, and PIAS1 heterohexamer ([C2B2P2]₂) binds to the three consecutive CP2c half-sites or two staggered CP2c binding motifs, where the [C4] ex

SUBMITTER: Son SH 

PROVIDER: S-EPMC9223585 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature