The Functional Characterization of GCaMP3.0 Variants Specifically Targeted to Subcellular Domains.

ABSTRACT: Calcium (Ca²⁺) ions play a pivotal role in physiology and cellular signaling. The intracellular Ca²⁺ concentration ([Ca²⁺]_i) is about three orders of magnitude lower than the extracellular concentration, resulting in a steep transmembrane concentration gradient. Thus, the spatial and the temporal dynamics of [Ca²⁺]_i are ideally suited to modulate Ca²⁺-mediated cellular responses to external signals. A variety of highly sophisticated methods have been developed to gain insight into cellular Ca²⁺ dynamics. In addition to electrophysiological measurements and the application of synthetic dyes that change their fluorescent properties upon interaction with Ca²⁺, the introduction and the ongoing de

SUBMITTER: Kempmann A 

PROVIDER: S-EPMC9223625 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature