Dataset Information

Transcriptomic Analysis and the Effect of Maturity Stage on Fruit Quality Reveal the Importance of the L-Galactose Pathway in the Ascorbate Biosynthesis of Hardy Kiwifruit (Actinidia arguta).

ABSTRACT: Hardy kiwifruit (Actinidia arguta) has recently become popular in fresh markets due to its edible skin and rich nutritional value. In the present study, different harvest stages of two A. arguta cultivars, 'Issai' and 'Ananasnaya' ("Ana"), were chosen for investigating the effects of maturity on the quality of the fruit. Interestingly, Issai contained 3.34 folds higher ascorbic acid (AsA) content than Ana. The HPLC method was used to determine the AsA content of the two varieties and revealed that Issai had the higher content of AsA and DHA. Moreover, RNA sequencing (RNAseq) of the transcriptome-based expression analysis showed that 30 differential genes for ascorbate metabolic pathways were screened in Issai compared to Ana, which had 16 genes down-regulated and 14 genes up-regulated, while compared to the up-regulation of 8 transcripts encoding the key enzymes involved in the L-galactose biosynthesis pathway. Our results suggested that AsA was synthesized mainly through the L-galactose pathway in hardy kiwifruit.

SUBMITTER: Lei D

PROVIDER: S-EPMC9223753 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Transcriptomic Analysis and the Effect of Maturity Stage on Fruit Quality Reveal the Importance of the L-Galactose Pathway in the Ascorbate Biosynthesis of Hardy Kiwifruit (Actinidia arguta).

Lei Diya D Lin Yuanxiu Y Chen Qiyang Q Zhao Bing B Tang Honglan H Zhang Yunting Y Chen Qing Q Wang Yan Y Li Mengyao M He Wen W Luo Ya Y Wang Xiaorong X Tang Haoru H Zhang Yong Y

International journal of molecular sciences 20220619 12

Hardy kiwifruit (Actinidia arguta) has recently become popular in fresh markets due to its edible skin and rich nutritional value. In the present study, different harvest stages of two A. arguta cultivars, 'Issai' and 'Ananasnaya' ("Ana"), were chosen for investigating the effects of maturity on the quality of the fruit. Interestingly, Issai contained 3.34 folds higher ascorbic acid (AsA) content than Ana. The HPLC method was used to determine the AsA content of the two varieties a ...[more]

PMID: 35743259

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Project description:Anthocyanin is the main component of pigment in red-fleshed kiwifruit. 'Jinhongguan' is a new cultivar of Actinidia arguta with red peel and flesh after harvest. However, the specific types of anthocyanin in the 'Jinhongguan' fruit and its biosynthesis pathways remain largely unknown. Here, the total anthocyanin content in the fruit color conversion process was determined. The results showed that total anthocyanin content increased with the deepening color of the peel and flesh. To identify the genes related to anthocyanin biosynthesis and the types of anthocyanins in the 'Jinhongguan' fruit, a combined analysis of transcriptome and anthocyanin-targeted metabolome was carried out. A total of 5751 common differentially expressed genes (DEGs) at different stages of peel and flesh were identified, of which 2767 were common up-DEGs and 2976 were common down-DEGs. KEGG and GO enrichment analyses showed that the common up-DEGs were significantly enriched in anthocyanin synthesis-related pathways, suggesting some up-DEGs are involved in anthocyanin biosynthesis. In total, 29 metabolites were detected in the flesh by anthocyanin-targeted metabolome. Among these, nine were differential accumulation metabolites (DAMs) in comparison to red flesh vs green flesh. Six DAMs were up-regulated, with five of them were cyanidins. The content of cyanidin-3-O-galactoside was much higher than that of other DAMs, making it the main pigment in 'Jinhongguan'. Moreover, a total of 36 anthocyanin synthesis-related structural genes, 27 MYB transcription factors (TFs), 37 bHLH TFs and 9 WDR TFs were screened from the common DEGs. Correlation analysis of transcriptome and metabolome revealed that 9 structural genes, 6 MYB TFs, 6 bHLH TFs and 1 WDR TF were significantly associated with cyanidin-3-O-galactoside. Further, qRT-PCR analysis demonstrated that structural genes (AaPAL3, Aa4CL3, AaCHS2/3/8/9/11, AaDFR1/2, AaANR1, UFGT3a and UFGT6b) and TFs (MYB108, bHLH30, bHLH94-1 and WD43) play important roles in cyanidin biosynthesis. Overall, this study identified cyanidin-3-O-galactoside as the main anthocyanin type and revealed key candidate genes of red coloration of post-harvest fruit in Actinidia arguta. These findings provided new insights into the color formation mechanism of post-harvest fruit and offered a theoretical basis for color regulation in kiwifruit.

Dataset Information

Transcriptomic Analysis and the Effect of Maturity Stage on Fruit Quality Reveal the Importance of the L-Galactose Pathway in the Ascorbate Biosynthesis of Hardy Kiwifruit (Actinidia arguta).

Publications

Transcriptomic Analysis and the Effect of Maturity Stage on Fruit Quality Reveal the Importance of the L-Galactose Pathway in the Ascorbate Biosynthesis of Hardy Kiwifruit (<i>Actinidia arguta</i>).

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