Exploring Honeybee Abdominal Anatomy through Micro-CT and Novel Multi-Staining Approaches.

ABSTRACT: Continuous improvements in morphological and histochemical analyses of Apis mellifera could improve our understanding of the anatomy and physiology of these insects at both the cellular and tissue level. In this work, two different approaches have been performed to add new data on the abdomen of worker bees: (i) Micro-computed tomography (Micro-CT), which allows the identification of small-scale structures (micrometers) with adequate/optimal resolution and avoids sample damage and, (ii) histochemical multi-staining with Periodic Acid-Schiff-Alcian blue, Lactophenol-Saphranin O and pentachrome staining to precisely characterize the histological structures of the midgut and hindgut. Micro-CT allowed high-resolution imaging of anatomical structures of the honeybee abdomen with particul

SUBMITTER: De Paula JC 

PROVIDER: S-EPMC9224579 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature