Paraphysoderma sedebokerense GlnS III Is Essential for the Infection of Its Host Haematococcus lacustris.

ABSTRACT: Glutamine synthetase (GlnS) is a key enzyme in nitrogen metabolism. We investigated the effect of the GlnS inhibitor glufosinate on the infection of H. lacustris by the blastocladialean fungus P. sedebokerense, assuming that interfering with the host nitrogen metabolism will affect the success of the parasite. Complete inhibition of infection, which could be bypassed by the GlnS product glutamine, was observed at millimolar concentrations of glufosinate. However, this effect of glufosinate was attributed to its direct interaction with the blastoclad and not the host, which results in development and growth inhibition of the blastoclad. In our P. sedebokerense draft genome, we found that the sequence of GlnS is related to another fungal GlnS, type III, found in many poo

SUBMITTER: Alors D 

PROVIDER: S-EPMC9224648 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature