Structural Analysis of Human Fascin-1: Essential Protein for Actin Filaments Bundling.

ABSTRACT: Fascin, a major actin cross-linking protein, is expressed in most vertebrate epithelial tissues. It organizes actin filaments into well-ordered bundles that are responsible for the extension of dynamic membrane protrusions, including microspikes, filopodia, and invadopodia from cell surfaces, which are involved in cell migration and invasion as critical components of cancer metastasis. However, it is not well-understood how fascin-1 induces actin binding/bundling and where fascin-1 localizes along the actin filaments, thus facilitating actin bundle formation. In the present study, we attempted to clarify these problems by using biochemical and electron microscopic analyses using various fascin-1 constructs. Three dimensional structures of actin/fascin-1 complex were obtained by electron mi

SUBMITTER: Chung JM 

PROVIDER: S-EPMC9224989 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature