Response of a soil bacterial community to grassland succession as monitored by 16S rRNA levels of the predominant ribotypes.

ABSTRACT: The composition of predominant soil bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural production at different time points, and one currently fertilized plot represented different stages of grassland succession. Since fertilization and agricultural production were stopped, the six plots showed a constant decline in the levels of nutrients and vegetation changes. The activity of the predominant bacteria was monitored by direct ribosome isolation from soil and temperature gradient gel electrophoresis of reverse transcription (RT)-PCR products generated from bacterial 16S rRNA. The amounts of 16S rRNA of 20 predominant ribosome types per gram of soil were monitored via multiple competitive RT-PCR in six plots at different

SUBMITTER: Felske A 

PROVIDER: S-EPMC92250 | biostudies-literature | 2000 Sep

REPOSITORIES: biostudies-literature