Genomic Analysis of Waterpipe Smoke-Induced Lung Tumor Autophagy and Plasticity.

ABSTRACT: The role of autophagy in lung cancer cells exposed to waterpipe smoke (WPS) is not known. Because of the important role of autophagy in tumor resistance and progression, we investigated its relationship with WP smoking. We first showed that WPS activated autophagy, as reflected by LC3 processing, in lung cancer cell lines. The autophagy response in smokers with lung adenocarcinoma, as compared to non-smokers with lung adenocarcinoma, was investigated further using the TCGA lung adenocarcinoma bulk RNA-seq dataset with the available patient metadata on smoking status. The results, based on a machine learning classification model using Random Forest, indicate that smokers have an increase in autophagy-activating genes. Comparative analysis of lung adenocarcinoma molecular signatures in affec

SUBMITTER: Zaarour RF 

PROVIDER: S-EPMC9225041 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature