M⁶A reader YTHDC1 modulates autophagy by targeting SQSTM1 in diabetic skin.

ABSTRACT: Dysregulation of macroautophagy/autophagy contributes to the delay of wound healing in diabetic skin. N⁶-methyladenosine (m⁶A) RNA modification is known to play a critical role in regulating autophagy. In this study, it was found that SQSTM1/p62 (sequestosome 1), an autophagy receptor, was significantly downregulated in two human keratinocyte cells lines with short-term high-glucose treatment, as well as in the epidermis of diabetic patients and a db/db mouse model with long-term hyperglycemia. Knockdown of SQSTM1 led to the impairment of autophagic flux, which was consistent with the results of high-glucose treatment in keratinocytes. Moreover, the m⁶A reader protein YTHDC1 (YTH domain containing 1), which interacted with SQSTM1 mRNA, was dow

SUBMITTER: Liang D 

PROVIDER: S-EPMC9225222 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature