Stable, efficient, and cost-effective system for the biosynthesis of recombinant bacterial cellulose in Escherichia coli DH5α platform.

ABSTRACT:

Background

Owing to its remarkable mechanical properties that surpass the plant-based cellulose, bacterial cellulose production has been targeted for commercialization during the last few years. However, the large-scale production of cellulose is generally limited by the slow growth of producing strains and low productivity which ultimately makes the commercial production of cellulose using the conventional strains non cost-effective. In this study, we developed a novel plasmid-based expression system for the biosynthesis of cellulose in E. coli DH5α and assessed the cellulose productivity relative to the typically used E. coli BL21 (DE) expression strain.

Results

No production was detected in BL21 (DE3) cultures upon expression induction; however, cellulose was detected in

SUBMITTER: Al-Janabi SS 

PROVIDER: S-EPMC9226222 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature