Binding of the transcription activator-like effector augments transcriptional regulation by another transcription factor.

ABSTRACT: DNA transcription is regulated by a range of diverse mechanisms and primarily by transcription factors that recruit the RNA polymerase complex to the promoter region on the DNA. Protein binding to DNA at nearby or distant sites can synergistically affect this process in a variety of ways, but mainly through direct interactions between DNA-binding proteins. Here we show that a Transcription Activator-Like Effector (TALE), which lacks an activation domain, can enhance transcription in mammalian cells when it binds in the vicinity of and without direct interaction with several different dimeric or monomeric transcription factors. This effect was observed for several TALEs regardless of the recognition sequences and their DNA-bound orientation. TALEs can exert an effect over the distance of te

SUBMITTER: Leben K 

PROVIDER: S-EPMC9226504 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature