Protoplast fusion in Bacillus species produces frequent, unbiased, genome-wide homologous recombination.

ABSTRACT: In eukaryotes, fine-scale maps of meiotic recombination events have greatly advanced our understanding of the factors that affect genomic variation patterns and evolution of traits. However, in bacteria that lack natural systems for sexual reproduction, unbiased characterization of recombination landscapes has remained challenging due to variable rates of genetic exchange and influence of natural selection. Here, to overcome these limitations and to gain a genome-wide view on recombination, we crossed Bacillus strains with different genetic distances using protoplast fusion. The offspring displayed complex inheritance patterns with one of the parents consistently contributing the major part of the chromosome backbone and multiple unselected fragments originating from the second parent. Our

SUBMITTER: Vasileva DP 

PROVIDER: S-EPMC9226520 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature