Metagenomic Screening for Lipolytic Genes Reveals an Ecology-Clustered Distribution Pattern.

ABSTRACT: Lipolytic enzymes are one of the most important enzyme types for application in various industrial processes. Despite the continuously increasing demand, only a small portion of the so far encountered lipolytic enzymes exhibit adequate stability and activities for biotechnological applications. To explore novel and/or extremophilic lipolytic enzymes, microbial consortia in two composts at thermophilic stage were analyzed using function-driven and sequence-based metagenomic approaches. Analysis of community composition by amplicon-based 16S rRNA genes and transcripts, and direct metagenome sequencing revealed that the communities of the compost samples were dominated by members of the phyla Actinobacteria, Proteobacteria, Firmicutes, Bacteroidetes, and Chlorof

SUBMITTER: Lu M 

PROVIDER: S-EPMC9226776 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature