Fast and Efficient Separation of Eleven Mycosporine-like Amino Acids by UHPLC-DAD and Their Quantification in Diverse Red Algae.

ABSTRACT: Due to their hostile habitats, characterized by a high exposure to UV-A and UV-B radiation, red algae are known to synthesize unique secondary metabolites: mycosporine-like amino acids (MAAs). These small molecules possess an extremely high UV absorption capacity and therefore mainly act as photoprotective agents. In this study, the first ultrahigh-performance liquid chromatography (UHPLC) method with diode-array detection (DAD) was developed for the determination of eleven MAAs in various algal species. All of the analytes could be separated in under 8 min on a Phenomenex Luna Omega C18 1.6 µm column. The mobile phase comprised water with 0.25% formic acid and 20 mM ammonium formate (A) and acetonitrile (B). Elution was carried out in gradient mode. Method validation following ICH guideli

SUBMITTER: Zwerger M 

PROVIDER: S-EPMC9227160 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature