New p35 (H3L) Epitope Involved in Vaccinia Virus Neutralization and Its Deimmunization.

ABSTRACT: Vaccinia virus (VACV) is a promising oncolytic agent because it exhibits many characteristic features of an oncolytic virus. However, its effectiveness is limited by the strong antiviral immune response induced by this virus. One possible approach to overcome this limitation is to develop deimmunized recombinant VACV. It is known that VACV p35 is a major protein for B- and T-cell immune response. Despite the relevance of p35, its epitope structure remains insufficiently studied. To determine neutralizing epitopes, a panel of recombinant p35 variants was designed, expressed, and used for mice immunization. Plaque-reduction neutralization tests demonstrated that VACV was only neutralized by sera from mice that were immunized with variants containing both N- and C- terminal regions of p35. Th

SUBMITTER: Khlusevich Y 

PROVIDER: S-EPMC9227246 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature