Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine.

ABSTRACT: Silica nanoparticles hold tremendous potential for the encapsulation of enzymes. However, aqueous alcohol solutions and catalysts are prerequisites for the production of silica nanoparticles, which are too harsh for maintaining the enzyme activity. Herein, a procedure without any organic solvents and catalysts (acidic or alkaline) is developed for the synthesis of silica-encapsulated glucose-oxidase-coated magnetic nanoparticles by a facile self-assembly route, avoiding damage of the enzyme structure in the reaction system. The encapsulated enzyme was characterized by scanning electron microscopy, transmission electron microscopy, energy-dispersive spectrometry, and a vibrating sample magnetometer. Finally, a colorimetric sensing method was developed for the detection of glucose in urine s

SUBMITTER: Luo Z 

PROVIDER: S-EPMC9227432 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature