Molecular phylogeny of Anabaena circinalis and its identification in environmental samples by PCR.

ABSTRACT: Although the cyanobacterium Anabaena circinalis occurs worldwide, Australian isolates are believed to exclusively possess the saxitoxin group neurotoxins (paralytic shellfish poisons). Identification of A. circinalis in a mixed population is complicated due to limited morphological differences between Anabaena species. Sequence analysis of the DNA-dependent RNA polymerase (rpoC1) gene from 24 Anabaena isolates, including 12 designated A. circinalis, permitted a phylogenetic analysis to be performed. In addition, an A. circinalis-specific PCR was developed and tested successfully on environmental samples.

SUBMITTER: Fergusson KM 

PROVIDER: S-EPMC92275 | biostudies-literature | 2000 Sep

REPOSITORIES: biostudies-literature