Metal-Organic Enzyme Nanogels as Nanointegrated Self-Reporting Chemobiosensors.

ABSTRACT: A fluorometric glucose biosensor based on fine-tuned chemoenzymatic nanohybrids is herein proposed. The successful integration of an engineered glucose oxidase enzyme and an optically responsive polymeric nanogel in a single entity has led to the fabrication of a highly efficient glucose chemobiosensor. The optical responsiveness has been achieved by the loading of preactivated polymeric hydrogel with fluorescent lanthanide, i.e., cerium (III), cations. A comprehensive investigation of the responsiveness of the biomaterial revealed the interplay between the oxidation state of the cerium lanthanide and the fluorescence emission of the polymer. Finally, a full structural, chemical, and biochemical characterization of the reported system supports the chemobiosensors as robust, specific, and s

SUBMITTER: Sanchez-deAlcazar D 

PROVIDER: S-EPMC9227723 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature