Simultaneous Quantitative Analysis of the Major Bioactive Compounds in Gentianae Radix and its Beverages by UHPSFC-DAD.

ABSTRACT: This study presents the first ultra-high performance supercritical fluid chromatography-diode array detector (UHPSFC-DAD) assay for simultaneous quantitation of secoiridoids, iridoids, xanthones, and xanthone glycosides in Gentiana lutea L. Separation was reached within 12 min on an Acquity UPC² BEH 2-EP column using CO₂ and methanol with 5.5% water as mobile phases. Method validation for nine selected marker compounds (gentisin, isogentisin, swertiamarin, sweroside, gentiopicroside, loganic acid, amarogentin, gentioside, and its isomer) confirmed the assay's sensitivity, linearity, precision, and accuracy. The practical applicability was proven by the analysis of 13 root specimens and 10 commercial liquid preparations (seven liqueurs and three clear spirits).

SUBMITTER: Gibitz-Eisath N 

PROVIDER: S-EPMC9228070 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature