Correlation between Polymerase Chain Reaction Identification of Iron Acquisition Genes and an Iron-Deficient Incubation Test for Klebsiella pneumoniae Isolates from Bovine Mastitis.

ABSTRACT: We investigated the correlation between the polymerase chain reaction (PCR) identification of six virulence genes associated with siderophore activation and the iron-uptake system (iron-acquisition genes; iucA, entB, fepA, ybtS, psn, and kfu) in mastitis-associated Klebsiella pneumoniae (K. pneumoniae). The growth of 37 K. pneumoniae isolates from the milk of cows with mild mastitis reared on Japanese dairy farms between October 2012 and December 2014 was examined by incubation in an iron-deficient medium. entB-, fepA-, or ybtS-positive isolates grew significantly better than entB-, fepA-, or ybtS-negative isolates after incubating in an iron-deficient medium for three days. Interesting

SUBMITTER: Tsuka T 

PROVIDER: S-EPMC9228167 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature