Reconstruction of the Genome-Scale Metabolic Model of Saccharopolyspora erythraea and Its Application in the Overproduction of Erythromycin.

ABSTRACT: Saccharopolyspora erythraea is considered to be an effective host for erythromycin. However, little is known about the regulation in terms of its metabolism. To develop an accurate model-driven strategy for the efficient production of erythromycin, a genome-scale metabolic model (iJL1426) was reconstructed for the industrial strain. The final model included 1426 genes, 1858 reactions, and 1687 metabolites. The accurate rates of the growth predictions for the 27 carbon and 31 nitrogen sources available were 92.6% and 100%, respectively. Moreover, the simulation results were consistent with the physiological observation and ¹³C metabolic flux analysis obtained from the experimental data. Furthermore, by comparing the single knockout targets with earlier published results, f

SUBMITTER: Xu F 

PROVIDER: S-EPMC9228414 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature