High Throughput Screening of Antimicrobial Resistance Genes in Gram-Negative Seafood Bacteria.

ABSTRACT: From a global view of antimicrobial resistance over different sectors, seafood and the marine environment are often considered as potential reservoirs of antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs); however, there are few studies and sparse results on this sector. This study aims to provide new data and insights regarding the content of resistance markers in various seafood samples and sources, and therefore the potential exposure to humans in a global One Health approach. An innovative high throughput qPCR screening was developed and validated in order to simultaneously investigate the presence of 41 ARGs and 33 MGEs including plasmid replicons, integrons, and insertion sequences in Gram-negative bacteria. Analysis of 268 seafood isolates from the bacterial mi

SUBMITTER: Delannoy S 

PROVIDER: S-EPMC9230514 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature