Viromes of Hungarian Peach Trees Identified by High-Throughput Sequencing of Small RNAs.

ABSTRACT: Peach trees can be infected with viruses and viroids. As we do not have efficient plant protection methods against these pathogens, the prevention of infection is crucial. Fruit trees are maintained by vegetative propagation. Planting material such as certified mother trees and rootstocks should be free from viruses and viroids, and this status has to be regularly checked to prevent infections. We surveyed certified peach trees for the presence of viruses and viroids using small RNA high-throughput sequencing (HTS), an unbiased virus diagnostic method. The results of the bioinformatic analysis of HTS were validated by other molecular methods including RT-PCR, Northern blot hybridization and loop-mediated isothermal amplification (LAMP). We found the presence of plum pox virus and peach lat

SUBMITTER: Barath D 

PROVIDER: S-EPMC9230589 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature