Developing Pseudovirus-Based Neutralization Assay against Omicron-Included SARS-CoV-2 Variants.

ABSTRACT: The global spread of SARS-CoV-2 and its variants poses a serious threat to human health worldwide. Recently, the emergence of Omicron has presented a new challenge to the prevention and control of the COVID-19 pandemic. A convenient and reliable in vitro neutralization assay is an important method for validating the efficiency of antibodies, vaccines, and other potential drugs. Here, we established an effective assay based on a pseudovirus carrying a full-length spike (S) protein of SARS-CoV-2 variants in the HIV-1 backbone, with a luciferase reporter gene inserted into the non-replicate pseudovirus genome. The key parameters for packaging the pseudovirus were optimized, including the ratio of the S protein expression plasmids to the HIV backbone plasmids and the collection time for the Al

SUBMITTER: Sun H 

PROVIDER: S-EPMC9231177 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature