Isotype-specific plasma cells express divergent transcriptional programs.

ABSTRACT: Antibodies are produced across multiple isotypes with distinct properties that coordinate initial antigen clearance and confer long-term antigen-specific immune protection. Here, we interrogate the molecular programs of isotype-specific murine plasma cells (PC) following helper T cell-dependent immunization and within established steady-state immunity. We developed a single-cell-indexed and targeted molecular strategy to dissect conserved and divergent components of the rapid effector phase of antigen-specific IgM⁺ versus inflammation-modulating programs dictated by type 1 IgG2a/b⁺ PC differentiation. During antibody affinity maturation, the germinal center (GC) cycle imparts separable programs for post-GC type 2 inhibitory IgG1⁺ and type 1 inflammatory IgG

SUBMITTER: Higgins BW 

PROVIDER: S-EPMC9231473 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature