Integrated screens uncover a cell surface tumor suppressor gene KIRREL involved in Hippo pathway.

ABSTRACT: Cell surface proteins play essential roles in various biological processes and are highly related to cancer development. They also serve as important markers for cell identity and targets for pharmacological intervention. Despite their great potentials in biomedical research, comprehensive functional analysis of cell surface proteins remains scarce. Here, with a de novo designed library targeting cell surface proteins, we performed in vivo CRISPR screens to evaluate the effects of cell surface proteins on tumor survival and proliferation. We found that Kirrel1 loss markedly promoted tumor growth in vivo. Moreover, KIRREL was significantly enriched in a separate CRISPR screen based on a specific Hippo pathway reporter. Further studies revealed that KIRREL binds directly to SAV1 to ac

SUBMITTER: Wang C 

PROVIDER: S-EPMC9231494 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature