Single-nucleus RNA-sequencing in pre-cellularization Drosophila melanogaster embryos.

ABSTRACT: Our current understanding of the regulation of gene expression in the early Drosophila melanogaster embryo comes from observations of a few genes at a time, as with in situ hybridizations, or observation of gene expression levels without regards to patterning, as with RNA-sequencing. Single-nucleus RNA-sequencing however, has the potential to provide new insights into the regulation of gene expression for many genes at once while simultaneously retaining information regarding the position of each nucleus prior to dissociation based on patterned gene expression. In order to establish the use of single-nucleus RNA sequencing in Drosophila embryos prior to cellularization, here we look at gene expression in control and insulator protein, dCTCF, maternal null embryos during zygotic genome acti

SUBMITTER: Albright AR 

PROVIDER: S-EPMC9232161 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature