O-glycosylation of the extracellular domain of pollen class I formins modulates their plasma membrane mobility.

ABSTRACT: In plant cells, linkage between the cytoskeleton, plasma membrane, and cell wall is crucial for maintaining cell shape. In highly polarized pollen tubes, this coordination is especially important to allow rapid tip growth and successful fertilization. Class I formins contain cytoplasmic actin-nucleating formin homology domains as well as a proline-rich extracellular domain and are candidate coordination factors. Here, using Arabidopsis, we investigated the functional significance of the extracellular domain of two pollen-expressed class I formins: AtFH3, which does not have a polar localization, and AtFH5, which is limited to the growing tip region. We show that the extracellular domain of both is necessary for their function, and identify distinct O-glycans attached to these sequences, At

SUBMITTER: Lara-Mondragon CM 

PROVIDER: S-EPMC9232206 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature