Viability markers for determination of desiccation tolerance and critical stages during dehydration in Selaginella species.

ABSTRACT: While most plants die below a threshold of water content, desiccation-tolerant species display specific responses that allow them to survive extreme dehydration. Some of these responses are activated at critical stages during water loss and could represent the difference between desiccation tolerance (DT) and death. Here, we report the development of a simple and reproducible system to determine DT in Selaginella species. The system is based on exposure of excised tissue to a dehydration agent inside small containers, and subsequent evaluation for tissue viability. We evaluated several methodologies to determine viability upon desiccation including: triphenyltetrazolium chloride (TTC) staining, the quantum efficiency of PSII, antioxidant potential, and relative electrolyte leakage. Our res

SUBMITTER: Alejo-Jacuinde G 

PROVIDER: S-EPMC9232207 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature