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Monoclonal antibodies binding data for SARS-CoV-2 proteins.

ABSTRACT: SARS-CoV-2 pandemic opens up the curiosity of understanding the coronavirus. This demand for the development of the regent, which can be used for academic and therapeutic applications. The present data provide the biochemical characterization of synthetically developed monoclonal antibodies for the SARS-CoV-2 proteins. The antibodies from phage-displayed antibody libraries were selected with the SARS-CoV-2 proteins immobilized in microwell plates. The clones which bind to the antigen in Fab-phage ELISA were selected, and a two-point competitive phage ELISA was performed. Antibodies binding kinetic of IgGs for SARS-CoV2 proteins further carried with B.L.I. Systematic analysis of binding with different control proteins and purified SARS-CoV-2 ensured the robustness of the antibodies.

SUBMITTER: Mishra N

PROVIDER: S-EPMC9232259 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Monoclonal antibodies binding data for SARS-CoV-2 proteins.

Mishra Nawneet N Teyra Joan J Boytz RuthMabel R Miersch Shane S Merritt Trudy N TN Cardarelli Lia L Gorelik Maryna M Mihalic Filip F Jemth Per P Davey Robert A RA Sidhu Sachdev S SS Leung Daisy W DW Amarasinghe Gaya K GK

Data in brief 20220624

SARS-CoV-2 pandemic opens up the curiosity of understanding the coronavirus. This demand for the development of the regent, which can be used for academic and therapeutic applications. The present data provide the biochemical characterization of synthetically developed monoclonal antibodies for the SARS-CoV-2 proteins. The antibodies from phage-displayed antibody libraries were selected with the SARS-CoV-2 proteins immobilized in microwell plates. The clones which bind to the antigen in Fab-phag ...[more]

PMID: 35789908

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