Nanopore sequencing of a monkeypox virus strain isolated from a pustular lesion in the Central African Republic.

ABSTRACT: Monkeypox is an emerging and neglected zoonotic disease whose number of reported cases has been gradually increasing in Central Africa since 1980. This disease is caused by the monkeypox virus (MPXV), which belongs to the genus Orthopoxvirus in the family Poxviridae. Obtaining molecular data is particularly useful for establishing the relationships between the viral strains involved in outbreaks in countries affected by this disease. In this study, we evaluated the use of the MinION real-time sequencer as well as different polishing tools on MinION-sequenced genome for sequencing the MPXV genome originating from a pustular lesion in the context of an epidemic in a remote area of the Central African Republic. The reads corresponding to the MPXV genome were identified using two taxonomic cla

SUBMITTER: Vandenbogaert M 

PROVIDER: S-EPMC9232561 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature