The validity evaluation of different 16srRNA gene primers for helicobacter detection urgently requesting to design new specific primers.

ABSTRACT: Molecular diagnosis of helicobacters by PCR is simpler, more accurate, and feasible compared to other diagnostic methods. Validity and accuracy are highly dependent on the PCR primer design, diffusion time, and mutation rate of helicobacters. This study aimed to design 16srRNA -specific primers for Helicobacter spp. and H. pylori. Application of comparative statistical analysis of the diagnostic utility of the most available 16srRNA genus-specific primers. The new primers were designed using bioinformatics tools (MAFFT MSA and Gblocks command line). A comparative study was applied on nine genus-specific 16srRNA primers in comparison to the ConsH using in silico and laboratory evaluation. The results demonstrated that the best specificity and sensitivity of the primers designed for this stu

SUBMITTER: Abdelmalek S 

PROVIDER: S-EPMC9232570 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature