In-frame editing of transcription factor gene RDD1 to suppress miR166 recognition influences nutrient uptake, photosynthesis, and grain quality in rice.

ABSTRACT: The transcription factor-encoding gene RDD1 increases the uptake of nutrient ions, photosynthetic activity under ambient and high CO₂ conditions, and grain productivity, and microRNA166 (miR166) regulates its transcript levels. This study found that CRISPR/Cas9 genome editing of rice plants to inhibit miR166-RDD1 transcript pairing (R1-Cas plants) increased RDD1 transcript levels, NH₄⁺ and PO₄^3- uptake, and photosynthetic activity under high CO₂ conditions in rice. However, the panicle weight of the R1-Cas plants decreased compared with the wild-type (WT) plants. Adversely, changes in environmental conditions, such as high CO₂ or high temperatures, showed insignificant differences in the panicle weight between the

SUBMITTER: Iwamoto M 

PROVIDER: S-EPMC9232572 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature