Electric-field facilitated rapid and efficient dissociation of tissues Into viable single cells.

ABSTRACT: Single-Cell Analysis is a growing field that endeavors to obtain genetic profiles of individual cells. Disruption of cell-cell junctions and digestion of extracellular matrix in tissues requires tissue-specific mechanical and chemical dissociation protocols. Here, a new approach for dissociating tissues into constituent cells is described. Placing a tissue biopsy core within a liquid-filled cavity and applying an electric field between two parallel plate electrodes facilitates rapid dissociation of complex tissues into single cells. Different solution compositions, electric field strengths, and oscillation frequencies are investigated experimentally and with COMSOL Multiphysics. The method is compared with standard chemical and mechanical approaches for tissue dissociation. Treatment of ti

SUBMITTER: Welch EC 

PROVIDER: S-EPMC9232619 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature