Imaging of innate immunity activation in vivo with a redox-tuned PET reporter.

ABSTRACT: High-redox-potential reactive oxygen species and reactive nitrogen species (ROS/RNS), generated by NADPH oxidase-2 (NOX2), myeloperoxidase (MPO) and related enzymes, are key effector molecules of innate immunity. High-redox-potential radicals are difficult to distinguish by imaging from less potent ROS/RNS functioning as background biological signaling molecules. Here we present 4-[¹⁸F]fluoro-1-naphthol ([¹⁸F]4FN), a redox-tuned radiopharmaceutical that selectively binds proteins and cells when oxidized by products of human MPO plus H₂O₂, but not H₂O₂ alone, and can be detected using positron emission tomography (PET). Activating HL-60 neutrophil-like human cells with phorbol ester (PMA) caused [¹⁸F]4FN retention

SUBMITTER: Pisaneschi F 

PROVIDER: S-EPMC9232999 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature