Simultaneous brain cell type and lineage determined by scRNA-seq reveals stereotyped cortical development.

ABSTRACT: Mutations are acquired frequently, such that each cell's genome inscribes its history of cell divisions. Common genomic alterations involve loss of heterozygosity (LOH). LOH accumulates throughout the genome, offering large encoding capacity for inferring cell lineage. Using only single-cell RNA sequencing (scRNA-seq) of mouse brain cells, we found that LOH events spanning multiple genes are revealed as tracts of monoallelically expressed, constitutionally heterozygous single-nucleotide variants (SNVs). We simultaneously inferred cell lineage and marked developmental time points based on X chromosome inactivation and the total number of LOH events while identifying cell types from gene expression patterns. Our results are consistent with progenitor cells giving rise to multiple cortical ce

SUBMITTER: Anderson DJ 

PROVIDER: S-EPMC9233029 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature