DOCK4 Regulation of Rho GTPases Mediates Pulmonary Vascular Barrier Function.

ABSTRACT:

Background

The vascular endothelium maintains tissue-fluid homeostasis by controlling the passage of large molecules and fluid between the blood and interstitial space. The interaction of catenins and the actin cytoskeleton with VE-cadherin (vascular endothelial cadherin) is the primary mechanism for stabilizing AJs (adherens junctions), thereby preventing lung vascular barrier disruption. Members of the Rho (Ras homology) family of GTPases and conventional GEFs (guanine exchange factors) of these GTPases have been demonstrated to play important roles in regulating endothelial permeability. Here, we evaluated the role of DOCK4 (dedicator of cytokinesis 4)-an unconventional Rho family GTPase GEF in vascular function.

Methods

We generated mice deficient in DOCK4' used DOCK4 si

SUBMITTER: Yazbeck P 

PROVIDER: S-EPMC9233130 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature