Extrusion: A New Method for Rapid Formulation of High-Yield, Monodisperse Nanobubbles.

ABSTRACT: Shell-stabilized gas microbubbles (MB) and nanobubbles (NB) are frequently used for biomedical ultrasound imaging and therapeutic applications. While it is widely recognized that monodisperse bubbles can be more effective in these applications, the efficient formulation of uniform bubbles at high concentrations is difficult to achieve. Here, it is demonstrated that a standard mini-extruder setup, commonly used to make vesicles or liposomes, can be used to quickly and efficiently generate monodisperse NBs with high yield. In this highly reproducible technique, the NBs obtained have an average diameter of 0.16 ± 0.05 µm and concentration of 6.2 ± 1.8 × 10¹⁰  NBs mL^-1 compared to 0.32 ± 0.1 µm and 3.2 ± 0.7 × 10¹¹  mL^-1 for NBs made using mechanical

SUBMITTER: Counil C 

PROVIDER: S-EPMC9233137 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature