MilliPillar: A Platform for the Generation and Real-Time Assessment of Human Engineered Cardiac Tissues.

ABSTRACT: Engineered cardiac tissues derived from human induced pluripotent stem cells (iPSCs) are increasingly used for drug discovery, pharmacology and in models of development and disease. While there are numerous platforms to engineer cardiac tissues, they often require expensive and nonconventional equipment and utilize complex video-processing algorithms. As a result, only specialized academic laboratories have been able to harness this technology. In addition, methodologies and tissue features have been challenging to reproduce between different groups and models. Here, we describe a facile technology (milliPillar) that covers the entire pipeline required for studies of engineered cardiac tissues. We include methodologies for (i) platform fabrication, (ii) cardiac tissue generation, (iii) ele

SUBMITTER: Tamargo MA 

PROVIDER: S-EPMC9233181 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature