Irisin Attenuates Pathological Neovascularization in Oxygen-Induced Retinopathy Mice.

ABSTRACT:

Purpose

Abnormal angiogenesis is a defining feature in a couple of ocular neovascular diseases. The application of anti-VEGFA therapy has achieved certain benefits in the clinic, accompanying side effects and poor responsiveness in many patients. The present study investigated the role of irisin in retinal neovascularization.

Methods

Western blot and quantitative PCR were used to determine irisin expression in the oxygen-induced retinopathy mice model. The pathological angiogenesis and inflammation index were examined after irisin administration. Primary retinal astrocytes were cultured and analyzed for VEGFA expression in vitro. Astrocyte-conditioned medium was collected for transwell assay and tube formation assay in human microvascular endothelial cells-1.

Results

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9233294 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature