Accurate recombination estimation from pooled genotyping and sequencing: a case study on barley.

ABSTRACT: Sexual reproduction involves meiotic recombination and the creation of crossing over between homologous chromosomes, which leads to new allele combinations. We present a new approach that uses the allele frequency differences and the physical distance of neighboring polymorphisms to estimate the recombination rate from pool genotyping or sequencing. This allows a considerable cost reduction compared to conventional mapping based on genotyping or sequencing data of single individuals. We evaluated the approach based on computer simulations at various genotyping depths and population sizes as well as applied it to experimental data of 45 barley populations, comprising 4182 RIL. High correlations between the recombination rates from this new pool genetic mapping approach and conventional mapp

SUBMITTER: Schneider M 

PROVIDER: S-EPMC9233355 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature