Agonist-induced Piezo1 activation promote mitochondrial-dependent apoptosis in vascular smooth muscle cells.

ABSTRACT:

Objective

Mechanical damage plays an essential role in the progression of atherosclerosis. Piezo1 is a new mechanically sensitive ion channel. The present study investigated the vascular smooth muscle cells (VSMCs) apoptosis induced by Piezo1 activation and explored its underlying mechanism.

Methods

We evaluated cell viability and apoptosis rate with cell counting kit-8 (CCK-8) and Annexin V-FITC/PI flow cytometry assay, respectively. And then Western blot was performed to measure the relative protein. Reactive oxygen species (ROS) and intracellular Ca²⁺ were assessed via fluorescence microscope, and the mitochondrial transmembrane potential was monitored by JC-10 staining.

Results

Our in vitro study revealed that mice in the ApoE-/- group compared with con

SUBMITTER: Yin Q 

PROVIDER: S-EPMC9233385 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature