Loss of starch synthase IIIa changes starch molecular structure and granule morphology in grains of hexaploid bread wheat.

ABSTRACT: Starch synthase III plays a key role in starch biosynthesis and is highly expressed in developing wheat grains. To understand the contribution of SSIII to starch and grain properties, we developed wheat ssIIIa mutants in the elite cultivar Cadenza using in silico TILLING in a mutagenized population. SSIIIa protein was undetectable by immunoblot analysis in triple ssIIIa mutants carrying mutations in each homoeologous copy of ssIIIa (A, B and D). Loss of SSIIIa in triple mutants led to significant changes in starch phenotype including smaller A-type granules and altered granule morphology. Starch chain-length distributions of double and triple mutants indicated greater levels of amylose than sibling controls (33.8% of starch in triple mutants, and 29.3% in double mutants vs. 25.5% in siblin

SUBMITTER: Fahy B 

PROVIDER: S-EPMC9233681 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature