Tyramide-conjugated DNA barcodes enable signal amplification for multiparametric CODEX imaging.

ABSTRACT: Multiparametric imaging allows researchers to measure the expression of many biomarkers simultaneously, allowing detailed characterization of cell microenvironments. One such technique, CODEX, allows fluorescence imaging of >30 proteins in a single tissue section. In the commercial CODEX system, primary antibodies are conjugated to DNA barcodes. This modification can result in antibody dysfunction, and development of a custom antibody panel can be very costly and time consuming as trial and error of modified antibodies proceeds. To address these challenges, we developed novel tyramide-conjugated DNA barcodes that can be used with primary antibodies via peroxidase-conjugated secondary antibodies. This approach results in signal amplification and imaging without the need to conjugate primary

SUBMITTER: Simonson PD 

PROVIDER: S-EPMC9234042 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature